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According to Chargaff's rule, base pairing in DNA should resemble this: A = T; C = G. Chargaff's rules states that DNA from any cell of all organisms should have a 1:1 ratio (base Pair Rule) of pyrimidine and purine bases. Hope this answers the question. Base Pairs in RNA. While RNA also conforms to Watson-Crick base pair rules, there are some key structural differences to note. There are examples of DNA that is single stranded, and RNA that is double stranded (i.e. RNAi) but typically, RNA is thought of as single-stranded and DNA as double stranded.

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